产品编号:3188-1

产品描述:Akt1 Phospho (pT450) antibody

反应种属:

实验方法:

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2850.00

订购:

说明书:

Akt1 Phospho (pT450) antibody

Cat.#: 3188-1

Rabbit Monoclonal Antibody

Description

Akt1/PKBa is a Serine/Threonine protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4).

Recommended Applications

WB, IHC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000 -10,000
IHC: 1:50 - 100
IP: 1:10 - 100

Species Reactivity

Human, Mouse, Rat

Products Data

Western blot analysis on NIH/3T3 cell lysate using anti-Akt1 Phospho (pT450) RabMAb (cat. #3188-1). Cells were either (A) untreated or (B) treated with Lambda Phosphatase.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Akt1 Phospho (pT450) RabMAb (cat. #3188-1).

  

Specificity

A phospho specific peptide corresponding to residues surrounding threonine 450 of human Akt1 was used as an immunogen. This antibody only detects Akt1 phosphorylated at threonine 450.

Storage Buffer & Conditions

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Description References

1. Burgering, B.M. and P.J. Coffer.Nature 376: 599-602 (1995).
2. Cardone, M.H., et al. Science 282: 1318-1321 (1998).
3. Hajduch, E., et al. FEBS Lett. 492: 199-203 (2001).
4. Cross, D.A., et al. Nature 378: 785-789 (1995).