产品编号:4508-1

产品描述:Akt1 (C-term)

反应种属:

实验方法:

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2750.00

订购:

说明书:

Akt1 (C-term) DyLight^® 488 antibody

Cat.#: 4508-1

Rabbit Monoclonal IgG conjugated with DyLight^® 488

Description

Akt1/PKBα is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBα is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBα inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBα regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBα isoforms have been identified.

Recommended Applications

ICC, FC

Applications and Recommended Dilution Factors

ICC: 1:100
FC: 1:50

Species Reactivity

Human

Products Data

Immunofluorescent analysis of A431 cells labeled with anti-Akt1 antibody (DyLight 488 conjugate, green) and Dapi (blue).

Flow cytometric analysis of Hela cells using Akt1 RabMAb (DyLight 488 conjugate) (red) compared to a negative control antibody (green).

  

Specificity

A synthetic peptide corresponding to residues in C-terminus of human Akt1 was used as immunogen. Predicted to cross-react with bovine based on sequence homology.

Storage Buffer & Conditions

Supplied in PBS (pH 7.2), 0.02% sodium azide, 0.05% BSA. Store at 4C. Protect from light. Stable for 6 months from date of receipt.

Description References

1. Burgering, B.M. and P.J. Coffer. Nature 376: 599–602 (1995). 2. Cardone, M.H., et al. Science 282: 1318–1321 (1998). 3. Hajduch, E., et al. FEBS Lett. 492: 199–203 (2001). 4. Cross, D.A., et al. Nature 378: 785–789 (1995).