产品编号:2548-1

产品描述:Adiponectin antibody

反应种属:

实验方法:

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

Adiponectin antibody

Cat.#: 2548-1

Rabbit Monoclonal Antibody

Description

Adiponectin is a multimeric adipocyte-specific secretory protein that regulates energy homeostasis, lipid metabolism, and insulin sensitivity with direct anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin predominantly inhibits proliferation of myelomonocytic lineage cells and is an important negative regulator in hematopoiesis and immune systems (1, 2). Adiponectin is reported to possibly function as an endogenous factor involved in lipid catabolism and storage or whole body metabolism (3). It is decreased in obesity and has been demonstrated to inhibit tumor necrosis factor-alpha (TNF-alpha)-induced expression of endothelial adhesion molecules. Adiponectin modulates the inflammatory response of endothelial cells, possibly through cross talk between cAMP-PKA and NF-kappaB signaling pathways (4). Adiponectin forms trimers, hexamers and 12- to 18-mers. The trimers are low molecular weight complexes (LMW). Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes (MMW)) and higher molecular weight (HMW) complexes (5).

Recommended Applications

WB, ICC

Applications and Recommended Dilution Factors

WB: 1:2,000 - 5,000
ICC: 1:100 - 250

Species Reactivity

Human

Products Data

A.Western blot analysis on (A) fetal fat and (B) human plasma lysates using anti-Adiponectin RabMAb (cat. #2548-1), dilution 1:5000.

B. Western blot analysis on human plasma lysates treated with (A) Beta-ME and heat, (B) heat only, (C) Beta-ME only, and (D) no treatment using anti-Adiponectin RabMAb (cat. #2548-1).

  

Specificity

A synthetic peptide corresponding to residues on human Adiponectin was used as an immunogen.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

ADIPOQ, ACDC, ACRP30, APM1, GBP28, Adiponectin, Gelatin-binding protein

Description References

1. Yokota T, et al. Blood 96(5):1723-32, 2000
2. Richards AA, et al. Mol Endocrinol. 20(7):1673-87, 2006
3. Nakano Y, et al. J Biochem. 120(4):803-12, 1996
4. Ouchi N, et al. Circulation. 102(11):1296-301, 2000
5. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)