产品编号:3293-1

产品描述:ADFP antibody

反应种属:

实验方法:

标记:

规格:100ul

供应商:Epitomics

价格(RMB):2500.00

订购:

说明书:

ADFP antibody

Cat.#: 3293-1

Rabbit Monoclonal Antibody

Description

ADFP (Adipose differentiation-related protein) is a major protein associated with the lipid droplets in various types of cells, including macrophage-derived foam cells and lipid-laden liver cells (1). The ADFP protein occurs only transiently in adipocytes. It is present at the surface of nascent lipid droplets in early stages of adipocyte differentiation but reduced dramatically after the onset of perilipin expression, whereas ADFP mRNA remains elevated (2). Up-regulation of ADFP may act to sequester fatty acids as triglycerides in discrete lipid droplets that could protect muscle from the detrimental effects of fatty acids on insulin action and glucose tolerance (3).

Recommended Applications

WB, ICC, FC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
ICC: 1:100 - 250
FC: 1:100 - 500

Species Reactivity

Human, Mouse, Rat

Products Data

Western blot analysis on (A) fetal liver, (B) HepG2, (C) JAR, and (D) human milk lysates using anti-ADFP RabMAb (cat. #3293-1).

Immunofluorescent staining of HepG2 cells using anti-ADFP RabMAb (cat. #3293-1).

Western blot analysis on (A) mouse brain and (B) rat brain lysates using anti-ADFP RabMAb (cat. #3293-1).

Specificity

A synthetic peptide corresponding to residues in human ADFP was used as an immunogen.

Storage Buffer & Conditions

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Description References

1. Masuda Y, et al. J Lipid Res 47(1):87-98, 2006
2. Sztalryd C, et al. J Biol Chem 281(45):34341-8, 2006
3. Phillips SA, et al. Obes Res 13(8):1321-9, 2005