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Recombinant Human sTNF RII

产品编号:T199-50

产品描述:Recombinant Human sTNF RII

反应种属:

实验方法:

标记:

规格:50ug

供应商:Leinco

价格(RMB):5175.00

订购:订购

说明书:

Recombinant Human Soluble Tumor Necrosis Factor RII (sTNF RII)
Purified No Carrier Protein
(TNFRSF1B, TNFR75, p75, CD120b, TNFR80, p80)
Prod. No.: T199
Source E. coli
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C

Description
Background:

TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-alpha but manifests a lower affinity to TNF-beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-alpha induced activities by acting as a decoy receptor. Recombinant human sTNFRII is an 18.9 kDa protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human sTNF RII is Mr 25 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Soluble Tumor Necrosis Factor Receptor Type II is determined by its inhibitory effect of the TNF-Alpha mediated cytotoxicity in mouse L-929 cells. The expected ED50 for this effect (in the presence of 0.25 ng/ml of recombinant human TNF-alpha) is 0.125 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

vpaqvvlt pykpepgyec qisqeyydrk aqmccakcpp gqyvkhfcnk tsdtvcadce asmytqvwnq frtclscsss cttdqveira ctkqqnrvca ceagrycalk thsgscrqcm rlskcgpgfg vassrapngn vlckacapgt fsdttsstdv crphricsil aipgnastda vcapesptls aiprtlyvsq peptrsqpld qepgpsqtps iltslgstpi ieqstkgg

Related Products
Anti-Human sTNF RII (Antigen Aff. Pur.) (Leinco Prod. No.: T476)
Anti-Mouse sTNF RII-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T490)