产品编号:T171-25
产品描述:Recombinant Human sTNF Receptor Type I
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):5100.00
订购:
说明书:
Recombinant Human Soluble Tumor Necrosis Factor Receptor Type I (sTNF Receptor Type I)
Purified No Carrier Protein
(TNFRSF1A, TNFAR, TNF-R55, TNFR60, p55, CD120a)
Prod. No.: T171
Source E. coli
Pkg. Size: 1 mg, 25 ug
Storage: -20°C to -70°C
Description
Background:
TNFR1 belongs to the TNFR superfamily of transmembrane proteins, and is expressed in most cell types. Binding of either TNF-alpha or TNF-beta to TNFR1 initiates a signal transduction pathway that results in the activation of the transduction factor NFkB, whose target genes are involved in the regulation of inflammatory responses, and, in certain cells induce apoptosis. Soluble TNF Receptor I (sTNFR1) is capable of inhibiting TNF-alpha and TNF-beta activities by acting as a decoy receptor that serves as a sink for the TNF ligands.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human sTNF Receptor Type I is Mr 21 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Soluble Tumor Necrosis Factor Receptor Type I is determined by its inhibitory effect of the TNF-Alpha mediated cytotoxicity in mouse L-929 cells. The expected ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-Alpha is 0.05 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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