产品编号:X111-1
产品描述:Recombinant Human XEDAR
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human X-linked Ectodysplasin Receptor (XEDAR)
Purified No Carrier Protein
((Ectodysplasin A2 Receptor) EDA2R, EDA-A2R, EDAA2R, TNFRSF27)
Prod. No.: X111
Source NSO Cells
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Background:
XEDAR (X-linked Ectodysplasin A2 Receptor) is a type II transmembrane and member of the TNF receptor superfamily. It is highly expressed during embryonic development and induces apoptosis (1). XEDAR is a receptor for Ectodysplasin-A2 (EDA-A2) which may be a promising agent for the gene therapy of osteosarcoma (2). It has also been associated with hypohidrotic ectodermal dysplasia (HED).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human XEDAR is Mr 41.8 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 61 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human XEDAR was determined by its ability to bind rhEDA-A2/His. Immobilized rhXEDAR/Fc at 1 μg/mL (100 μL/well) can bind rhEDA-A2/His with a linear range of 0.045 - 5 ng/mL in a functional ELISA.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mdcqeneywd qwgrcvtcqr cgpgqelskd cgygeggday ctacpprryk sswghhrcqs citcavinrv qkvnctatsn avcgdclprf yrktrigglq dqecipctkq tptsevqcaf qlslveadap tvppqedieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgk
References
1. Preet, M. et al. (2004) J. Biol. Chem. 279:41873
2. Chaudhary, P. M. et al. (2007) Cancer Gene Ther. 14:927
Related Products
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