产品编号:W156-2
产品描述:Recombinant Mouse WNT-3a
反应种属:
实验方法:
标记:
规格:2ug
供应商:Leinco
价格(RMB):3630.00
订购:
说明书:
Recombinant Mouse WNT-3a
Purified No Carrier Protein
(Wingless-Type MMTV Integration Site Family, Member 3A, MGC119418, MGC119419, MGC119420)
Prod. No.: W156
Source CHO
Pkg. Size: 2 µg
Storage: -20°C to -70°C
Description
Background:
Wingless-type MMTV integration site family, member 3A, also known as WNT3A is an intercellular signaling molecule.1 WNT3A is expressed extensively in cells fated to give rise to embryonic mesoderm.2 Wnt-3a regulates survival, expansion, and maintenance of neural progenitors derived from embryonic stem cells.3 It has been reported that Wnt-3a supports self-renewal of embryonic stem cells and can maintain these cells in a pluripotent state.4 Wnt-3a is also considered a ligand that activates the beta-catenin-dependent pathway in Wnt signaling, which is implicated in numerous physiological events such as morphogenesis.5
Source
CHO
Molecular Weight
The predicted molecular weight of Recombinant Mouse WNT-3a is Mr 37 kDa. However, because of glycosylation the actual molecular weight as observed by migration on SDS Page is Mr 41 kDa.
State of Matter
Lyophilized
Purity
>75% as determined by SDS-PAGE and visualized by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse WNT-3a was determined by its ability to synergize with BMP-2 to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells (Fischer, L. et al., 2002, J. Biol. Chem. 277:30870). The expected ED50 is typically 2 - 10 ng/mL, in the presence of a low or non-inducing amount of recombinant human BMP-2 (5 - 15 ng/mL). Approximately 50 - 100 ng/mL results in maximal synergy with BMP-2 activity.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
A DNA sequence encoding the signal peptide from human CD33, Met 1 - Ala 16, joined with the mature mouse Wnt-3A, Ser 19 - Lys 352 was inserted into a suitable mammalian expression vector and expressed in CHO cells.
References
1. Li GW et al. (2007) Neurol Res. 29: 847
2. McMahon AP et al. (1994) Genes Dev. 8: 174
3. Davidson KC et al. (2007) Molecular Cellular Neurosci. 36: 408
4. Singla DK et al. (2006) Biochem Biophys Res Communications 354: 789
5. Kitagawa H et al. (2008) J Biol Chem. 283: 27333
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