产品编号:W106-20
产品描述:Recombinant Human WISP-2
反应种属:
实验方法:
标记:
规格:20ug
供应商:Leinco
价格(RMB):5205.00
订购:
说明书:
Recombinant Human WNT-1 Inducible Signaling Pathway Protein-2 (WISP-2)
(Connective Tissue Growth Factor-Like Protein (CTGFL), CCN5, CT58)
Prod. No.: W106
Source E. Coli
Pkg. Size: 1 mg, 20 µg
Description
Background:
WNT1 inducible signaling pathway protein 2, also known as WISP2 is a secreted protein that induces estrogen1 and is a member of the CCN family of growth factors.2 WISP-2 is an important regulator involved in the maintenance of a differentiated phenotype in breast tumor epithelial cells and may play a role in tumor cell invasion and metastasis.3
Source
E. Coli
State of Matter
Purity
>95% by SDS Page and HPLC
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg
Biological Activity
The biological activity of Human WISP-2 is determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF
References
1. Matsushima K et al. (2002) Biochem Biophys Res Commun. 294: 602
2. Michèle Sabbah et al. (2006) J Endocrin. 191: 613
3. Sabbah M et al. (2008) Mol Cell Biol. 28: 1114
Related Products
Recombinant Human WNT-1 (Leinco Prod. No.: W108)
Anti-Human WISP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C344)
Anti-Human WISP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: C345)
