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Recombinant Human WISP-1

产品编号:W105-1

产品描述:Recombinant Human WISP-1

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:订购

说明书:

Recombinant Human WNT-1 Inducible Signaling Pathway Protein-1 (WISP-1)
Purified No Carrier Protein
(CCN4, WISP1c, WISP1i, WISP1tc)
Prod. No.: W105
Source NSO Cells
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C 

Description
Background:

WNT1 inducible signaling pathway protein 1 (WISP-1), also known as CCN4, is a secreted, heparin-binding glycoprotein belonging to the cysteine-rich CCN family (1-2). WISP-1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. It is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival (3). WISP-1 promotes mesenchymal cell proliferation and osteoblastic differentiation while repressing chondrocytic differentiation. Therefore, WISP-1 plays an important regulatory role during bone development and fracture repair (4). It was reported that WISP-1 interacts with sulfated glycoconjugates, decorin and biglycan in the ECM of connective tissue, and possibly prevents their inhibitory activity in tumor cell proliferation (5). WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein (5). Mutated or spliced variants of WISP-1 have been implicated in various pathological conditions.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human WISP-1 is Mr 39.3 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 62 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human WISP-1 was determined in a cell proliferation assay using NRK49F rat fibroblasts. Xu, L. et al. (2000) Gene and Dev. 14:585. The expected ED50 is 1.5 - 7.5 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

talspapt tmdftpaple dtssrpqfck wpcecppspp rcplgvslit dgcecckmca qqlgdnctea aicdphrgly cdysgdrpry aigvcaqvvg vgcvldgvry nngqsfqpnc kynctcidga vgctplclrv rpprlwcphp rrvsipghcc eqwvceddak rprktaprdt gafdavgeve awhrnciayt spwspcstsc glgvstrisn vnaqcwpeqe srlcnlrpcd vdihtlikag kkclavyqpe asmnftlagc istrsyqpky cgvcmdnrcc ipyksktidv sfqcpdglgf srqvlwinac fcnlscrnpn difadlesyp dfseianhhh hhhhhhh

References
1. Tchou-Wong, K. M. et al. (2003) J. Bio.l Chem. 278:11465
2. Levine, A. J. et al. (2002) Genes Dev. 16:46
3. Pennica, D. et al. (1998) Proc. Nat. Acad. Sci. (USA) 95:14717
4. Desnoyers, L. et al. (2004) Am. J. Pathol. 165:855
5. Levine, A. J. et al. (2000) Genes Dev. 14:585
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