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Recombinant Mouse VEGF R2

产品编号:V120-50

产品描述:Recombinant Mouse VEGF R2

反应种属:

实验方法:

标记:

规格:50ug

供应商:Leinco

价格(RMB):4800.00

订购:订购

说明书:

Recombinant Mouse Vascular Endothelial Growth Factor Receptor 2 (VEGF R2)
Purified No Carrier Protein
(KDR, Flk-1, CD309, VEGFR)
Prod. No.: V120
Source NSO Cells
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C

Description
Background:

Vascular endothelial growth factor receptor 2 (VEGF R2) is a transmembrane glycoprotein that is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration, and differentiation.1,2,3

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse VEGF R2 is Mr 110 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 166-191 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse VEGF R2 was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The expected ED50 for this effect is typically 10 - 30 ng/ml in the presence of 5 ng/ml of rmVEGF.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

a svglpgdflh ppklstqkdi ltilanttlq itcrgqrdld wlwpnaqrds eervlvtecg ggdsifcktl tiprvvgndt gaykcsyrdv diastvyvyv rdyrspfias vsdqhgivyi tenknktvvi pcrgsisnln vslcarypek rfvpdgnris wdseigftlp symisyagmv fceakindet yqsimyivvv vgyriydvil sppheielsa geklvlncta rtelnvgldf twhsppsksh hkkivnrdvk pfpgtvakmf lstltiesvt ksdqgeytcv assgrmikrn rtfvrvhtkp fiafgsgmks lveatvgsqv ripvkylsyp apdikwyrng rpiesnytmi vgdeltimev terdagnytv iltnpismek qshmvslvvn vppqigekal ispmdsyqyg tmqtltctvy anpplhhiqw ywqleeacsy rpgqtspyac kewrhvedfq ggnkievtkn qyaliegknk tvstlviqaa nvsalykcea inkagrgerv isfhvirgpe itvqpaaqpt eqesvsllct adrntfenlt wyklgsqats vhmgesltpv cknldalwkl ngtmfsnstn dilivafqna slqdqgdyvc saqdkktkkr hclvkqliil ermapmitgn lenqtttige tievtcpasg nptphitwfk dnetlvedsg ivlrdgnrnl tirrvrkedg glytcqacnv lgcaraetlf iiegaqektn leiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh

References
1. Homqvist K et al. (2004) J Biol Chem. 279: 22267
2. Meyer R.D et al. (2002) J Biol Chem. 277: 27081
3. Rahimi N et al. (2000) J Biol Chem. 275: 16986
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