产品中心

产品搜索 抗体高级搜索

首页 > 产品列表 > 细胞因子/重组蛋白/抗原 > 重组与天然蛋白质

Recombinant Human TRAIL/Apo2L

产品编号:T164-1

产品描述:Recombinant Human TRAIL/Apo2L

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:订购

说明书:

Recombinant Human TNF-Related Apoptosis Inducing Ligand/Apo2 Ligand (TRAIL/Apo2L)
Purified No Carrier Protein
(TNFSF10, TL2)
Prod. No.: T164
Source E. coli
Pkg. Size: 1 mg, 10 µg

Description
Background:

TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain,” which activates the cells apoptotic machinery. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human TRAIL/Apo2L is Mr 19 kDa.
State of Matter
Lyophilized
Purity
>98% by SDS-PAGE and HPLC
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human TNF-Related Apoptosis Inducing Ligand/Apo2 Ligand is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG