产品编号:T220-5
产品描述:Recombinant Mouse Thrombopoietin(TPO)
反应种属:
实验方法:
标记:
规格:5ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Mouse Thrombopoietin (TPO)
Purified No Carrier Protein
(THPO, MGC163194, Megakaryocyte Growth and Development Factor (MGDF), MKCSF, ML, MPLLG, MpI Ligand, Leukemia Virus Oncogene Ligand)
Prod. No.: T220
Source NSO Cells
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse TPO is Mr 35 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 80-90 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse TPO was determined by a cell proliferation assay using MO7e cells (Avanzi, G. et al., 1988, Br. J. Haematol. 69:359). The expected ED50 for this effect is typically 1 - 3 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
sspvapacdp rllnkllrds hllhsrlsqc pdvdplsipv llpavdfslg ewktqteqsk aqdilgavsl llegvmaarg qlepsclssl lgqlsgqvrl llgalqgllg tqlplqgrtt ahkdpnalfl slqqllrgkv rflllvegpt lcvrrtlptt avpsstsqll tlnkfpnrts glletnfsvt artagpglls rlqgfrvkit pgqlnqtsrs pvqisgylnr thgpvngthg lfagtslqtl easdispgaf nkgslafnlq gglppspsla pdghtpfpps palptthgsp pqlhplfpdp sttmpnstap hpvtmyphpr nlsqet
References
1. Vainchenker W et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda RC et al. (1998) Blood 92: 4023
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