产品编号:T155-1
产品描述:Recombinant Human TPO
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Thrombopoietin (TPO)
Purified No Carrier Protein
(THPO, MGC163194, Megakaryocyte Growth and Development Factor (MGDF), MKCSF, ML, MPLLG, MpI Ligand, Leukemia Virus Oncogene Ligand)
Prod. No.: T155
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human TPO is Mr 35 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 47 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 20% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Thrombopoietin is determined by the dose-dependent stimulation of the proliferation of human MO7e cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
spappacdl rvlskllrds hvlhsrlsqc pevhplptpv llpavdfslg ewktqmeetk aqdilgavtl llegvmaarg qlgptclssl lgqlsgqvrl llgalqsllg tqlppqgrtt ahkdpnaifl sfqhllrgkv rflmlvggst lcvrrapptt avpsrtslvl tlnelpnrts glletnftas arttgsgllk wqqgfrakip gllnqtsrsl dqipgylnri hellngtrgl fpgpsrrtlg apdissgtsd tgslppnlqp gyspspthpp tgqytlfplp ptlptpvvql hpllpdpsap tptptsplln tsythsqnls qeg
References
1. Vainchenker W et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda RC et al. (1998) Blood 92: 4023
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