产品编号:T390-50
产品描述:Recombinant Human TNF sRI
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Human Soluble Tumor Necrosis Factor Receptor I (TNF sRI)
Purified No Carrier Protein
(TNFRSF1A, P55, TBP1, CD120a, FPF, MGC19588, TNF-R, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55-R, p60)
Prod. No.: T390
Source NSO Cells
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated.2 TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.2 It regulates immune and inflammatory responses.4
Source
NSO Cells
Molecular Weight
The predicted molecular weight of monomeric Recombinant Human TNF sRI is Mr 48 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE under reducing conditions is Mr 60-66 kDa. TNF sRI is expressed in NSO cells as a disulfide-linked homodimer.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human TNF sRI was determined by measuring its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The expected ED50 for this effect is approximately 0.4 - 1.0 ng/ml in the presence of 0.25 ng/ml rhTNF-α1.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
(lvphlgdrekr) dsvcpqgkyi hpqnnsicct kchkgtylyn dcpgpgqdtd crecesgsft asenhlrhcl scskcrkemg qveissctvd rdtvcgcrkn qyrhywsenl fqcfncslcl ngtvhlscqe kqntvctcha gfflrenecv scsnckksle ctklclpqie vkgtedsgtt niegrmdksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgkhhh hhh
References
1. Neale M.L et al. (1987) in Lymphokines & Interferons, A Practical Approach. Clemens, M.J. et al.:IRL Press.221
2. Reza Dana et al. (2000) Arch Ophthalmol. 118: 1666
3. Donner DB et al. (2008) J Immunol. 181: 1288
4. Pasparakis M et al. (2008) Nat Immunol. 9: 1015
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