产品编号:T170-1
产品描述:Recombinant Human TGF-β1
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Transforming Growth Factor-Beta 1 (TGF-β1)
Purified No Carrier Protein
(TGFB, DPD1, TGFB1, Differentiation Inhibiting Factor, Cartilage-Inducing Factor, Latency Associated Peptide (LAP), CED, DPD1)
Prod. No.: T170
Source CHO
Pkg. Size: 1 mg, 2 µg, 10 µg
Storage: -20°C to -70°C
Description
Background:
Transforming growth factor beta 1 or TGF-β1 is a multifunctional cytokine that regulates the proliferation and differentiation of cells specific to bone like chondrocytes, osteoblasts, osteoclasts including mesenchymal precursor cells.1 It is known to promote the pathogenesis of lung fibrosis and acts as a tumor suppressor in normal cells.2 TGF-beta1 can promote mechanical strength in healing Achilles tendons by regulating collagen synthesis, cross-link formation, and matrix remodeling.3
Source
CHO
Molecular Weight
The predicted molecular weight of Recombinant Human TGF-β1 is Mr 25 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Transforming Growth Factor-Beta 1 (mammalian derived) is determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 for this effect is 0.04 - 0.2 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the human TGF-β1 precursor protein (Derynck, R. et al., 1985, Nature 316:701 - 705) was inserted into a suitable mammalian expression vector and expressed in CHO cells.
References
1. Guven M et al. (2007) Growth Factors 25: 101
2. Schneider J et al. (2009) J Investing Med.
3. Yu C et al. (2009) Biochem Biophys Res Commun.
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