产品编号:R133-50
产品描述:Recombinant Human RANTES
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):9900.00
订购:
说明书:
Recombinant Human Regulation Upon Activation Normal T Cell Expressed Sequence (RANTES)
Purified No Carrier Protein
(CCL5, D17S136E, MGC17164, SCYA5, SIS-Delta, TCP228)
Prod. No.: R133
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
RANTES also known as Chemokine (C-C motif) ligand 5 (CCL5), a CC-chemokine1 is an important mediators of the immune and inflammatory response.2 It is also a potent chemoattractant for T cells 3 and eosinophils.4 RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayed-type hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.5
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human RANTES is Mr 7.8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human RANTES was determined by its ability to chemoattract human monocytes (Schall, T. et al., 1990, Nature 347:669 - 671) or mouse BaF/3 cells transfected with human CCR5. The expected ED50 for these effects are typically 20 - 30 ng/ml and 1 - 5 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
spyssdt tpccfayiar plprahikey fytsgkcsnp avvfvtrknr qvcanpekkw vreyinslem s
References
1. Lee SC et al. (2004) J Neurochem. 90: 297
2. Borkowski J et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201
3. Geiger H et al. (2005) Kidney Blood Press Res 28: 48
4. Hirata I. et al. (2008) Dig Dis Sci.
5. Emilie D et al. (1994) J Exp Med. 179: 1689
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