产品编号:R1258-1
产品描述:Recombinant Human RANK
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Receptor Activator of NF-κB (RANK)
Purified No Carrier Protein
(Osteoclast Differentiation Factor Receptor [ODFR], TNFRSF11A, CD265, OFE, RANK, TRANCER)
Prod. No.: R1258
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
RANK (Receptor Activator of Nuclear Factor κ B), also known as TRANCE Receptor is a type I membrane protein which is TNF receptor family member1 expressed on the surface of osteoclasts and is involved in the activation of osteoclasts upon ligand binding. It is also expressed on dendritic cells and facilitates immune signaling. RANK is highly expressed in mature osteoclasts.2 RANK is the intrinsic cell surface determinant that mediates osteoprotegerin ligand effects on bone resorption and remodeling as well as the physiological and pathological effects of calciotropic hormones and proresorptive cytokines.3
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human RANK is Mr 48 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 60 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human RANK was determined by its ability to inhibit TRANCE-induced osteoclast differentiation on mouse splenocytes. The expected ED50 for this effect is typically 0.1 - 0.25 μg/ml in the presence of 10 ng/ml of Recombinant Mouse TRANCE.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mqi appctsekhy ehlgrccnkc epgkymsskc tttsdsvclp cgpdeyldsw needkcllhk vcdtgkalva vvagnsttpr rcactagyhw sqdceccrrn tecapglgaq hplqlnkdtv ckpclagyfs dafsstdkcr pwtnctflgk rvehhgteks davcssslpa rkppnephvy lpgiegrdmd pkscdkthtc ppcpapellg gpsvflfppk pkdtlmisrt pevtcvvvdv shedpevkfn wyvdgvevhn aktkpreeqy nstyrvvsvl tvlhqdwlng keykckvsnk alpapiekti skakgqprep qvytlppsrd eltknqvslt clvkgfypsd iavewesngq pennykttpp vldsdgsffl yskltvdksr wqqgnvfscs vmhealhnhy tqkslslspg khhhhhh
References
1. Aggarwal BB et al. (1998) J Biol Chem. 273: 20551
2. Nicholson GC et al. (1999) FEBS Lett. 463: 295
3. Boyle WJ et al. (2000) Proc Natl Acad Sci USA 97: 1566
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