产品编号:P167-1
产品描述:Recombinant Human PlGF-1
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Placenta Growth Factor-1 (PlGF-1)
Purified No Carrier Protein
(PGFL, PLGF, PlGF-2, D12S1900, SHGC-10760, PGF)
Prod. No.: P167
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Placenta growth factor 1 (PlGF-1), also known as PLGF, is a member of the vascular endothelial growth factor (VEGF) family and a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis (1). PlGF and VEGF share primary structure similarities but limited amino acid sequence homology. The expression of PlGF-1 is not widespread but has been detected in HUV endothelial cells, placenta and mammary carcinomas. The biologically active form of this protein is a disulfide-linked dimer. PlGF heterodimers are mitogens for vascular endothelial cells and are nearly as potent as VEGF homodimers, while PlGF homodimers are mitogenic for endothelial cells only at high, possibly non-physiologic concentrations. PlGF has a high affinity to Flt-1 but not to Flt-1/KDR (2). Expression of PlGF within human atherosclerotic lesions is associated with plaque inflammation and neovascular growth (3). In addition, recent studies have shown that PlGF-1 may be a potential regulation target for the control of diabetic retinal and macular oedema (4). PlGF deficiency diminishes and abbreviates inflammatory responses, and reduces inflammatory angiogenesis and edema formation (5).
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human PlGF-1 is Mr 14.5 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 14 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human PlGF-1 was determined by its ability to bind Recombinant Human Flt-1 in a functional ELISA. Immobilized Recombinant Human Flt-1 at 2 μg/ml (100 μl/well) can bind Recombinant Human PlGF-1 (Leinco Prod. No.: P167) with a linear range at 0.1 - 5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
avppqqwals agngssevev vpfqevwgrs ycralerlvd vvseypseve hmfspscvsl lrctgccgde nlhcvpveta nvtmqllkir sgdrpsyvel tfsqhvrcec rplrekmkpe rcgdavprr
References
1. Cao, Y. et al. (1997) Biochem. Biophy. Res. Comm. 235:493
2. Luttun, A. et al. (2003) Ann. N. Y. Acad. Sci. 979:80
3. Pilarczyk, K. et al. (2008) Atherosclerosis 196:333
4. Behar-Cohen, F. et al. (2007) Diabetologia 50:461
5. Oura, H. et al. (2003) Blood 101:560
Related Products
Anti-Human PlGF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: P175)
Anti-Human PlGF-1 (Antigen Aff. Pur.) (Leinco Prod. No.: R174)
