产品编号:P250-50
产品描述:Recombinant Rat Prolactin Receptor(PRL-R)
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Rat Prolactin Receptor (PRL-R)
Purified No Carrier Protein
(RATPRLR, MGC105486)
Prod. No.: P250
Source NSO Cells
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Prolactin Receptor (PRLR) is a cytokine receptor superfamily member that lacks an intrinsic kinase domain and relies on the cytoplasmic Jak tyrosine kinases to transduce signals (1). Prolactin receptors have been localized in a number of peripheral tissues, including tissues involved in reproduction (2). Within the immune system, multiple isoforms of the prolactin receptor serve to mediate the effects of its ligand prolactin. These isoforms are structurally and functionally distinct, causing significant differences in ligand affinities, kinetics of transduction and the transduction proteins activated. Differential activation of these pathways may contribute to the pleiotropism of prolactin action in tissues of the immune system (3). Prolactin receptor is required for normal maternal glucose homeostasis during pregnancy (4).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Rat PRL is Mr 51 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 67-74 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Rat PRL was determined by Measured by its ability to neutralize the rhProlactin-induced proliferation of the rat lymphoma Nb2-11 treated with 0.5 ng/mL of rhProlactin. (Gout, P.W. et al., 1980, Cancer Research 40:2433 - 2436). The expected ED50 for this effect is typically 0.005 - 0.02 mg/mL in the presence of 0.5 mg/mL of rhProlactin.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
q sppgkpeihk crspdketft cwwnpgtdgg lptnysltys kegekttyec pdyktsgpns cffskqytsi wkiyiitvna tnqmgssssd plyvdvtyiv epepprnltl evkqlkdkkt ylwvkwsppt itdvktgwft meyeirlkpe eaeeweihft ghqtqfkvfd lypgqkylvq trckpdhgyw srwsqessve mpndftlkdd iegrmdpksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgk
References
1. Lindeman, G. J. et al. (2003) J. Biol. Chem. 278:32929
2. Makrigiannakis, A. et al. (2005) Reproductive Biomed. 10:339
3. Clevenger, C. V. et al. (2001) Lupus 10:706
4. Mastro, M. et al. (2003) J. Appl. Physiol. 94:518
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