产品编号:P244-50
产品描述:Recombinant Mouse Prolactin(PRL)
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):4275.00
订购:
说明书:
Recombinant Mouse Prolactin (PRL)
Purified No Carrier Protein
(Mammotropin, Luterotropic Hormone (LTH), Lutetropin)
Prod. No.: P244
Source E. coli
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Prolactin (PRL) or Luteotropic hormone (LTH) is best known as the pituitary modulator of lactation and reproduction (1). PRL is a multifaceted hormone that is capable of modulating hundreds of physiological processes in adult vertebrates (2). It is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, NK cells and breast cancer cells. PRL promotes proliferation, survival and migration of cancer cells acting via the prolactin receptor (PRLR) (3). It also modulates maternal behavior and mediates hypothalamic pituitary adrenal axis inhibition during lactation via PRL receptors in the brain (4). PRL also has a number of other effects including contributing to surfactant synthesis of the fetal lungs at the end of the pregnancy and immune tolerance of the fetus by the maternal organism during pregnancy. It plays an additional immune-regulatory role by stimulating the activities of ornithine decarboxylase and protein kinase C, which are important for the proliferation, differentiation, and function of lymphocytes. PRL also decreases normal levels of sex hormones, estrogen in women and testosterone in men.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse PRL is Mr 22.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse PRL was determined by its ability to induce proliferation of the rat lymphoma Nb2-11 (Gout, P.W. et al., 1980, Cancer Research 40:2433 - 2436). The expected ED50 for this effecty is typically 0.25 - 1 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mlpicsagdc qtslrelfdr vvilshyiht lytdmfiefd kqyvqdrefm vkvindcpts slatpedkeq alkvppevll nlilslvqss sdplfqlitg vggiqeapey ilsrakeiee qnkqllegve kiisqaypea kgngiyfvws qlpslqgvde eskilslrnt irclrrdshk vdnflkvlrc qiahqnnc
References
1. Freeman, M. et al. (2000) Physiological Reviews 80:153
2. Ben-Johnson, N. et al. (1996) Endoc. Rev. 17:639
3. Cesario, T. et al. (1994) Proc. Soc. Exp. Biol. Med. 205:89
4. Price, A. E. et al. (1995) Endoc. 136:4827
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