产品编号:O101-1
产品描述:Recombinant Human Oncostatin M(OSM)
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Oncostatin M (OSM)
Purified No Carrier Protein
(MGC20461)
Prod. No.: O101
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Oncostatin M (OSM) is a pleitropic cytokine produced by activated T lymphocytes and monocytes that is structurally and functionally related to the subfamily of cytokines known as the IL-6-type cytokine family.1 OSM either inhibits or enhances the growth of cells depending on cell type. It inhibits proliferation of skeletal muscle cells by blocking cell cycle progression from G(1) to S phase.2 OSM is also a key mediator for proliferation and anti-apoptosis of hepatocytes which suggests that OSM gene therapy is useful, as preventive and curative means, for the treatment of patients with liver damage.3
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human OSM is Mr 22 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human OSM was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323-334). The expected ED50 for this effect is typically 0.15 - 0.3 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
aaigscskey rvllgqlqkq tdlmqdtsrl ldpyiriqgl dvpklrehcr erpgafpsee tlrglgrrgf lqtlnatlgc vlhrladleq rlpkaqdler sglniedlek lqmarpnilg lrnniycmaq lldnsdtaep tkagrgasqp ptptpasdaf qrklegcrfl hgyhrfmhsv grvfskwges pnrsrr
References
1. Gómez-Lechón MJ. et al. (1999) Life Sci. 65: 2019
2. Jo SA. et al. (2008) Cell Signal 20: 120
3. Tsujimura T. et al. (2007) Am J Pathol. 171: 872
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