产品编号:O122-1
产品描述:Recombinant Mouse Osteoprotegerin(OPG)
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse Osteoprotegerin(OPG)
Purified No Carrier Protein
(TNFRSF11B, Osteoclast Inhibitory Factor (OCIF), MGC29565, TR1, OCIT)
Prod. No.: O122
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) is a cytokine, which can inhibit osteoclast differentiation, activity, and survival.1 It is a member of the tumor necrosis factor (TNF) receptor superfamily.2 OPG may provide a pharmacological tool for osteoporotic and erosive bone disorders.3
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse OPG is Mr 70.9 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 85-95 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse OPG was determined by its ability to inhibit TRAIL mediated cytotoxicity using L929 mouse fibrosarcoma cells treated with TRAIL. The expected ED50 for this effect is 25-50 ng/ml in the presence of 50 ng/ml of recombinant human TRAIL.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
etlppkylh ydpetghqll cdkcapgtyl kqhctvrrkt lcvpcpdhsy tdswhtsdec vycspvckel qsvkqecnrt hnrvceceeg ryleiefclk hrscppgsgv vqagtpeqnt vckkcpdgff sgetsskapc rkhtncstfg llliqkgnat hdnvcsgnre atqkcgidvt lceeaffrfa vptkiipnwl svlvdslpgt kvnaesveri krrhssqeqt fqllklwkhq nrdqemvkki iqdidlcess vqrhlghanl tteqlralme slpgkkispe eiertrktck sseqllklls lwrikngdqd tlkglmyalk hlktshfpkt vthslrktmr flhsftmyrl yqklflemig nqvqsvkisc liegrmdpks cdkthtcppc papellggps vflfppkpkd tlmisrtpev tcvvvdvshe dpevkfnwyv dgvevhnakt kpreeqynst yrvvsvltvl hqdwlngkey kckvsnkalp apiektiska kgqprepqvy tlppsrdelt knqvsltclv kgfypsdiav ewesngqpen nykttppvld sdgsfflysk ltvdksrwqq gnvfscsvmh ealhnhytqk slslspgkhh hhhh
References
1. Kurihara H et al. (2002) Cytokine 18: 133
2. Schoppet M et al. (1980) Arteriosclerosis, Thrombosis, & Vascular Biol. 22: 549
3. Dunstan CR et al. (2001) J Bone Miner Res. 16: 348
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