产品编号:O102-25
产品描述:Recombinant Human OPG
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):5475.00
订购:
说明书:
Recombinant Human Osteoprotegerin (OPG)
Purified No Carrier Protein
(TNFRSF11B, Osteoclast Inhibitory Factor (OCIF), MGC29565, TR1, OCIT)
Prod. No.: O102
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Osteoprotegerin (OPG), also known as TNFRSF11B, is a cytokine and member of the TNF receptor superfamily that is expressed in a wide variety of tissues including adult heart, lung, kidney, liver, spleen, prostate, lymph node and bone marrow. OPG is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption (1). It specifically binds to its ligand, osteoprotegerin ligand (OPGL), which is a key extracellular regulator of osteoclast development. Therefore, OPG may provide a pharmacological tool for osteoporotic and erosive bone disorders (2-3). Studies also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification (4).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of each monomer of mature OPG (generated after removal of a 21 amino acid signal peptide) is Mr 43.5 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page under reducing conditions, as a result of glycosylation, of recombinant Human OPG is Mr 55 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human OPG was determined by its ability to neutralize apoptosis of mouse L-929 cells treated with 20 ng/ml cross-linked soluble rhTRAIL. The expected ED50 for this effect is typically 8 - 24 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
etfppkylh ydeetshqll cdkcppgtyl kqhctakwkt vcapcpdhyy tdswhtsdec lycspvckel qyvkqecnrt hnrvceckeg ryleiefclk hrscppgfgv vqagtpernt vckrcpdgff snetsskapc rkhtncsvfg llltqkgnat hdnicsgnse stqkcgidvt lceeaffrfa vptkftpnwl svlvdnlpgt kvnaesveri krqhssqeqt fqllklwkhq nkaqdivkki iqdidlcens vqrhighanl tfeqlrslme slpgkkvgae diektikack psdqilklls lwrikngdqd tlkglmhalk hsktyhfpkt vtqslkktir flhsftmykl yqklflemig nqvqsvkisc l
References
1. Schoppet, M. et al. (1980) Arteriosclerosis, Thrombosis, & Vascular Biol. 22:549
2. Kurihara, H. et al. (2002) Cytokine 18:133
3. Dunstan, C. R. et al. (2001) J. Bone Miner. Res. 16:348
4. Collin-Osdoby, P. et al. (2004) Circulation Res. 95:1046
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