产品编号:N182-1
产品描述:Recombinant Mouse Noggin(Nog)
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse Noggin (Nog)
Purified No Carrier Protein
(SYM1, SYNS1)
| Prod. No.: |
N182 |
| Source |
NSO Cells |
| Pkg. Size: |
1 mg, 50 µg |
| Storage: |
-20°C to -70°C |
Description
Background:
Noggin is a secreted glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis that inhibits TGF-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin is expressed in condensing cartilage and immature chondrocytes.1 Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Noggin plays a crucial role in bone development, joint formation, and neural tube fusion.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse Noggin/Fc Chimera is Mr 50 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 55 - 60 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse Noggin/Fc Chimera was determined by its ability to inhibit rhBMP-4 induced alkaline phosphatase production by ATDC5 cells. The expected ED50 for this effect is typically 0.06 - 0.3 μg/ml in the presence of 75 ng/ml of rhBMP-4.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the signal peptide from human CD33 joined with amino acid residues 20 - 232 of mouse noggin was fused to the Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NS0.
