产品编号:N142-20
产品描述:Recombinant Human Noggin(Nog)
反应种属:
实验方法:
标记:
规格:20ug
供应商:Leinco
价格(RMB):5025.00
订购:
说明书:
Recombinant Human Noggin(Nog)
Purified No Carrier Protein
(SYM1, SYNS1)
Prod. No.: N142
Source E. coli
Pkg. Size: 1 mg, 20 µg
Storage: -20°C to -70°C
Description
Background:
Noggin is a secreted glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis that inhibits TGF-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin is expressed in condensing cartilage and immature chondrocytes.1 Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Noggin plays a crucial role in bone development, joint formation, and neural tube fusion.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human Noggin is Mr 23.1 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS Page and HPLC
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Noggin was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 µg/ml of NOGGIN.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
References
1. Harland RM. et al. (1998) Science 280: 1455
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