产品编号:N118-1
产品描述:Recombinant Human NT-4
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Neurotrophin-4 (NT-4)
Purified No Carrier Protein
(Neurotrophin 5, NT5, NTF5, Neutrophic Factor 5, Neutrophic Factor 4)
Prod. No.: N118
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Neurotrophin 4 (NT-4), also known as NT-5, is a member of the NGF family and is related to β-NGF, BDNF and NT-3 (1). It promotes the development and survival of selected peripheral and CNS neurons. Several aspects distinguish NT-4 from other members of the neurotrophin family. It is the most divergent member and, in contrast to the other neurotrophins, its expression is ubiquitous and appears to be less influenced by environmental signals. NT-4 levels are highest in the prostate, with lower levels in thymus, placenta and skeletal muscle. It signals predominantly through the TrkB receptor tyrosine kinase. Knock-out mice lacking expression of NT-4 show long-term memory defects (3). In addition, NT-4 promotes the proliferation of keratinocytes and accelerates hair follicle regression (4). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (5).
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human NT-4 is Mr 14 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human NT-4 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED50 for this effect is typically 5 - 15 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
gvsetapasr rgelavcdav sgwvtdrrta vdlrgrevev lgevpaaggs plrqyffetr ckadnaeegg pgaggggcrg vdrrhwvsec kakqsyvral tadaqgrvgw rwiridtacv ctllsrtgra
References
1. Persson, H. et al. (1993) Development 117:1345
2. Ibanez, C. F. et al. (1996) Neurochem. Sci. 21:787
3. Xie, C. W. et al. (2000) Proc. Natl. Acad. Sci. (USA) 97:8116
4. Botchkarev, V. A. et al. (1999) Lab. Invest. 79:557
5. Laurenzi, M. A. et al. (1988) J. Leukoc. Biol.64:228
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