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Recombinant Human NT-3

产品编号:N117-25

产品描述:Recombinant Human NT-3

反应种属:

实验方法:

标记:

规格:25ug

供应商:Leinco

价格(RMB):9900.00

订购:订购

说明书:

Recombinant Human Neurotrophin-3 (NT-3)
Purified No Carrier Protein
(Nerve Growth Factor-2 (NGF-2), HGN NTF3, HDNF, MGC129711)
Prod. No.: N117
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C

Description
Background:

Neurotrophin 3 (NT-3), also known as NGF-2 and HDNF, is a basic protein and member of the NGF family. It is structurally related to β-NGF, BDNF and NT-4 (1), is expressed by neurons of the central nervous systems and can signal through the Trk receptors (2). NT-3 is also expressed in muscles and its expression is down-regulated in denervated muscles. It rapidly potentiates the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses (3). NT-3 negatively modulates NGF receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. In addition, NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI) (4). NT-3 affects development of neurons in the embryo when it is expressed in human placenta. Mice born without the ability to make NT-3 show the loss of pro-prioceptive including subsets of mechanoreceptive sensory neurons (5). The amino acid sequences of human, murine and rat NT-3 are identical.

Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human NT-3 is Mr 13.6 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human NT-3 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED50 for this effect is typically 10 - 30 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

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References
1. Barde, Y. A. et al. (1990) Prog. Growth Factor Res. 2:237
2. Tessarollo, L. et al. (1993) Development 118:463
3. Maisonpierre, P. C. et al. (1990) Science 247:1446
4. Valerie, M. K. et al. (2005) J. Neuroscie. 25:758
5. Tessarollo, L. et al. (1994) Proc. Natl. Acad. Sci. (USA) 91:11844
Related Products
Anti-Human NT-3 (Antigen Aff. Pur.) (Leinco Prod. No.: N113)
Anti-Human NT-3 (Leinco Prod. No.: N200)
Anti-Human NT-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N128)