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Recombinant Human MMP-2

产品编号:M1251-1

产品描述:Recombinant Human MMP-2

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:订购

说明书:

Recombinant Human Matrix Metalloproteinase-2 (MMP-2)
Purified No Carrier Protein
(CLG4, CLG4A, MMP-II, MONA, TBE-1)
Prod. No.: M1251
Source NSO Cells
Pkg. Size: 1 mg, 10 µg
Storage: -20°C to -70°C

Description
Background:

Matrix metallopeptidase 2, also known as MMP2, is an enzyme involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-2 degrades type IV collagen, the major structural component of basement membranes and plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. MMP2 has been found to be associated with tumor invasion in various cancers.1

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human MMP-2 is Mr 71 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 69 kDa.
State of Matter
Lyophilized
Formulation
The protein was 0.2 μm filtered and lyophilized from a solution of 25 mM Mes, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 5.5.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MMP-2 was determined by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl) Acetyl-Pro-Leu-Gly-Leu- (3-[2, 4-dinitrophenyl-L-2, 3-diaminopropionyl)-Ala-Arg-NH2. The specific activity, measured under the described conditions, is > 1,000 pmoles/min/μg.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

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References
1. Melissa Schorr et al. (2005) Medscape Medical News
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