产品编号:M180-25
产品描述:Recombinant Human MIP-3
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Human CCL23 (MIP-3)
Purified No Carrier Protein
(SCYA23, Ckb-8, Myeloid Progenitor Inhibitory Factor-1 (MPIF-1), CKb8, HMRP-2A (Human MIP-Related Protein 2A))
Prod. No.: M180
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Chemokine (C-C motif) ligand 23 (CCL23), also known as MPIF-1, is a small cytokine belonging to the CC chemokine family (1). It is most closely related to CCL15. CCL23 is predominantly expressed in lung and liver tissue but is also found in bone marrow, placenta and cells of the myeloid lineage (2). CCL23 induces endothelial cell migration and tube formation. It also enhances the expression of MMP-2 mRNA and protein levels in endothelial cells. CCL23 may play a direct role in angiogenesis (3). It is highly chemotactic for resting T cells and monocytes and slightly chemotactic for neutrophils and has also been attributed to an inhibitory activity on hematopoietic progenitor cells (2). CCL23 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human MIP-3 is Mr 11.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 10.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Macrophage Inflammatory Protein-3 is determined by its ability to chemoattract human T cell population using a concentration of 10.0-50.0 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
rvtkdaete fmmsklplen pvlldrfhat sadccisytp rsipcslles yfetnsecsk pgvifltkkg rrfcanpsdk qvqvcmrmlk ldtriktrkn
References
1. Huang, S. A. et al. (2007) J. Exp. Hematol. 15:496
2. Patel, V. et al. (1997) J. Exp. Med. 185:1163
3. Woisetschläger, M. et al. (2007) J. Immunol 178: 4335
4. Kim, J. et al. (2006) Biochem. Biophy. Res. Comm. 340:498
