产品编号:M169-25
产品描述:Recombinant Mouse MIP-1γ
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Mouse Macrophage Inflammatory Protein-1 Gamma (MIP-1γ)
Purified No Carrier Protein
(CCL9, CCF18, Macrophage Inflammatory Protein-Related Protein-2 (MRP2), CCL10, SCYB9)
Prod. No.: M169
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Macrophage inflammatory protein 1 gamma (MIP-1 gamma) is a monokine with inflammatory, pyrogenic, and chemokinetic CC properties. MIP-1 gamma plays an important role in the differentiation and survival of osteoclasts.1
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse MIP-1γ is Mr 11.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse MIP-1γ was determined by its ability to chemoattract mouse BaF/3 cells transfected with hCCR-1 and by its ability to chemoattract human THP-1 cells. The expected ED50 for these effects are typically 0.002 - 0.01 μg/ml and 0.2 - 0.6 μg/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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References
1. Stashenko P.et al. (2004) J. Immunol. 173: 2084
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