产品编号:M161-1
产品描述:Recombinant Human MIP-1α
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α)
Purified No Carrier Protein
(G0S19-1, LD78Alpha, MIP1A, SCYA3, CCL3)
Prod. No.: M161
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Macrophage inflammatory protein 1 alpha (MIP-1α), also known as CCL3, is a member of the chemokine family (1). Its primary function is as a monocyte chemoattractant, but reports have also demonstrated chemoattractant and adhesive effects by MIP-1α on CD8 T cells, B cells and eosinophils. MIP-1α has been identified as a stem cell inhibitory factor that can block the proliferation of hematopoietic stem cells (2). The functional receptor for MIP-1α is CCR1 and CCR5 and it is has been shown to interact with CCL4 (3). Following acute lung injury in rats, MIP-1α stimulates production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx (4). MIP-1α may have a potential role in host defense as a direct antiviral agent. It has been reported to show a significant direct antiviral activity against HSV-1(5).
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human MIP-1α is Mr 7.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein solutiion is supplied as a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MIP-1α was determined by its ability to chemoattract cultured human monocytes or mouse BaF3 cells transfected with human CCR5. The expected ED50 for these effects are typically 2 - 10 ng/ml and 3 - 10 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
adtp taccfsytsr qipqnfiady fetssqcskp gvifltkrsr qvcadpseew vqkyvsdlel sa
References
1. Menten, P. et al. (2003) Cytokine Growth Factor Rev. 13:455
2. Cook, D. N. et al. (1996) J. Leuko. Biol. 59:61
3. Kaufmann, A. et al. (2001) J. Leuko. Biol. 69:248
4. Ward, P. A. et al. (1995) J. Immunol. 154:4793
5. Nakayama, T. et al. (2006) Virology 350:484
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