产品编号:M202-1
产品描述:Recombinant Mouse CCL22
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse Chemokine (C-C Motif) Ligand 22 (CCL22)
Purified No Carrier Protein
(MDC, A-152E5.1, ABCD-1, DC/B-CK, MGC34554, SCYA22, STCP-1)
Prod. No.: M202
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemoattract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once thes cells have migrated into tissue.4
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse CCL22 is Mr 7.8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse MDC was determined by its ability to chemoattract human T-lymphoblastoid CEM-NKR cells (Howell, D.N. et al., 1985, J. Immunol. 134: 971 - 976) and to chemoattract mouse BaF/3 cells transfected with hCCR4. The expected ED50 for these effects are typically 5 - 20 ng/ml and 0.5 - 3 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
gpyganveds iccqdyirhp lpsrlvkeff wtskscrkpg vvlitvknrd icadprqvwv kkllhkls
References
1. Sozzani S. et al. (2001) Eur. J. Immunol. 31: 812
2. Elias CG. et al. (1998) J Immunol. 161: 5027
3. Gray PW. et al. (1997) J Exp Med. 185: 1604
4. Teixeira MM. et al. (2003) J Leuko Biol. 73: 356
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