产品编号:M134-10
产品描述:Recombinant Mouse MCP-3
反应种属:
实验方法:
标记:
规格:10ug
供应商:Leinco
价格(RMB):3750.00
订购:
说明书:
Recombinant Mouse Monocyte Chemotactic Protein-3 (MCP-3)
Purified No Carrier Protein
(CCL7, SCYA6, SCYA7, Small Inducible Cytokine A7, NC28, Chemokine (C-C Motif) Ligand 7, FIC, MARC, MGC138463, MGC138465)
Prod. No.: M134
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.1 MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes.
MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.2 MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.3,4,5
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse MCP-3 is Mr 8.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse MCP-3 was determined by its ability to chemoattract 2-day cultured human monocytes (Matsushima, K. et al., 1989, J. Exp. Med. 169:1485) and its ability to chemoattract mouse BaF/3 cells transfected with hCCR2A. The expected ED50 for these effects are typically 0.1 - 0.3 μg/ml and 0.5 - 2 μg/ml respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
qpdgpna stccyvkkqk ipkrnlksyr ritssrcpwe avifktkkgm evcaeahqkw veeaiayldm ktptpkp
References
1. Van Damme J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535
2. Parmentier M. et al. (1999) Blood 94: 1899
3. Brosnan CF. et al. (1998) J Neuroimmunol. 86: 20
4. Reddigari SR. et al. (1995) Exp. Dermatol. 4: 260
5. Matikainen S. et al. (2001) Cytokine Growth Factor Rev. 12: 171
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