产品编号:M156-10
产品描述:Recombinant Human MCP-1
反应种属:
实验方法:
标记:
规格:10ug
供应商:Leinco
价格(RMB):3000.00
订购:
说明书:
Recombinant Human Monocyte Chemotactic Protein-1 (MCP-1)
Purified No Carrier Protein
(CCL2, GDCF-2, GDCF-2 HC11, HC11, HSMCR30, MCAF, MGC9434, SCYA2, SMC-CF, JE)
Prod. No.: M156
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Monocyte chemotactic protein-1 (MCP-1) is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.4
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human MCP-1 is Mr 8.7 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MCP-1 was determined by its monocyte chemotactic activity and its ability to chemoattract hCCR2A transfected mouse BaF/3 cells. The expected ED50 for these effects are typically 5 - 20 ng/ml and 10 - 60 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
qpdaina pvtccynftn rkisvqrlas yrritsskcp keavifktiv akeicadpkq kwvqdsmdhl dkqtqtpkt
References
1. Yoshimura T. et al. (1989) J. Exp. Med. 169: 1449
2. Oppenheim J.J. et al. (1989) J. Exp. Med. 169: 1485
3. Rollins B.J. et al. (1997) Blood 90: 909
4. Benjamin EJ. et al. (2005) Ciculation 112: 1113
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