产品编号:M1098-50
产品描述:Recombinant Mouse M-CSF
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):9900.00
订购:
说明书:
Recombinant Mouse Macrophage Colony Stimulating Factor (M-CSF)
Purified No Carrier Protein
(CSF-1, MGI-IM, MGC31930, Csfm, C87615)
Prod. No.: M1098
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.1 It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.2
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse M-CSF is Mr 26 kDa (monomer). However, the actual molecular weight of the biologically active disulfide-linked homodimeric protein as observed by migration on SDS Page is Mr 52 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse M-CSF was determined by a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS-60 (Halenbeck, R. et al., 1989, Biotechnology 7:710 - 715). The expected ED50 for this effect is typically 0.5 - 1.5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mkevsehcs hmignghlkv lqqlidsqme tscqiafefv dqeqlddpvc ylkkafflvq diidetmrfk dntpnanate rlqelsnnln scftkdyeeq nkacvrtfhe tplqllekik nffnetknll ekdwniftkn cnnsfakcss rdvvtkpdcn clypkatpss dpasasphqp papsmaplag lawddsqrte gssllpselp lriedpgsak qrpprstcqt le
References
1. Nemunaitis J. et al. (1998) Clin. Infect. Dis. 26: 1279
2. Ko JS. et al. (2002) Exp Mol Med 34: 340
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