产品编号:M155-1
产品描述:Recombinant Human M-CSF
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Macrophage Colony Stimulating Factor (M-CSF)
Purified No Carrier Protein
(CSF-1, MGI-IM, MGC31930)
Prod. No.: M155
Source E. coli
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20 to -70°C
Description
Background:
Macrophage colony-stimulating factor (M-CSF), also known as CSF-1, is a secreted cytokine and key regulator of cellular proliferation, differentiation and survival of blood monocytes, tissue macrophages and their progenitor cells. It is produced by monocytes, fibroblasts and endothelial cells and binds to the CSF-1 receptor, also known as CD115. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis and secondary cytokine production in monocytes and macrophages (1). M-CSF also primes macrophage killing of tumor cells and microorganisms and stimulates pinocytosis. It is known as one of the factors essential for osteoclast development (2). M-CSF has been shown to play important roles in modulating dermal thickness and growth of the decidua and placenta during pregnancy (3). It is clinically used in the treatment of infection, malignancies and atherosclerosis, as well as, facilitating hematopoietic recovery after bone marrow transplantation (4).
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human M-CSF is Mr 18.5 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 37 kDa.
State of Matter
Solution
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human M-CSF was determined in a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS-60 (Halenbeck, R. et al., 1989, Biotechnology 7:710 - 715). The expected ED50 for this effect is 0.5 - 1.5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
meevseycs hmigsghlqs lqrlidsqme tscqitfefv dqeqlkdpvc ylkkafllvq dimedtmrfr dntpnaiaiv qlqelslrlk scftkdyeeh dkacvrtfye tplqllekvk nvfnetknll dkdwnifskn cnnsfaecss qgherqsegs
References
1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26:1279
2. Ko, J. S. et al. (2002) Exp. Mol. Med. 34:340
3. Makrigiannakis, A. et al. (2006) Trends Endicrinol. Metab. 17:178
4. Van de Pol, C. J. et al. (1991) Biotechnol. Therap. 2:231
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