产品编号:L116-5
产品描述:Recombinant Human Leptin
反应种属:
实验方法:
标记:
规格:5mg
供应商:Leinco
价格(RMB):7725.00
订购:
说明书:
Recombinant Human Leptin
Purified No Carrier Protein
(Obesity Protein (OB), B219, LEP, OBS)
Prod. No.: L116
Source E. coli
Pkg. Size: 1 mg, 5 mg
Storage: -20°C to -70°C
Description
Background:
Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.1 It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.2 High leptin levels are associated with psoriasis.3
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human Leptin is Mr 16 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Leptin was determined by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BaF3 cells. The expected ED50 for this effect is 0.4 - 2 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mvpiqkvqdd tktliktivt rindishtqs vsskqkvtgl dfipglhpil tlskmdqtla vyqqiltsmp srnviqisnd lenlrdllhv lafskschlp wasgletlds lggvleasgy stevvalsrl qgslqdmlwq ldlspgc
References
1. Mantzoros CS et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318
2. Hill RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407
3. Chen Y-J. et al.(2008) Arch Derm. 144: 1571
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