产品编号:L205-25
产品描述:Recombinant Human LBP
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Human Lipopolysaccharide-Binding Protein (LBP)
Purified No Carrier Protein
(MGC22233)
Prod. No.: L205
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Lipopolysaccharide binding protein, also known as LBP, is a soluble acute phase secretory protein that binds to bacterial lipopolysaccharide (LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4.1 LBP is a major mediator of the lethal effects of endotoxemia.2
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human LBP is Mr 51 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55-60 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 10mM Tris, 75 mM NaCl, and EDTA pH 7.5.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human LBP was determined by its ability to enhance LPS stimulated IL-8 secretion by THP1 human acute monocytic leukemia cells. The expected ED50=0.25 - 2.5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
anpgl varitdkglq yaaqegllal qsellritlp dftgdlriph vgrgryefhs lnihscellh salrpvpgqg lslsisdssi rvqgrwkvrk sffklqgsfd vsvkgisisv nlllgsessg rptvtasscs sdiadvevdm sgdlgwllnl fhnqieskfq kvlesricem iqksvssdlq pylqtlpvtt eidsfadidy slveaprata qmlevmfkge ifhrnhrspv tllaavmslp eehnkmvyfa isdyvfntas lvyheegyln fsitddmipp dsnirlttks frpfvprlar lypnmnlelq gsvpsaplln fspgnlsvdp ymeidafvll pssskepvfr lsvatnvsat ltfntskitg flkpgkvkve lkeskvglfn 421 aelleallny yilntlypkf ndklaegfpl pllkrvqlyd lglqihkdfl flganvqymr 481 v
References
1. Takeshige K. et al.(2001) Eur. J. Biochem. 268: 4580
2. Grauser MP. et al.(1993) Proc Natl Acad Sci U S A. 90: 9935
Related Products
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