产品编号:L203-1
产品描述:Recombinant Human L-Selectin
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human L-Selectin
Purified No Carrier Protein
(SELL, CD62L, LAM-1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc)
Prod. No.: L203
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain1 found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.2
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human L-Selectin is Mr 60.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 90-100 kDa under reducing conditions.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS Page and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells, a human colon adenocarcinoma cell line. When 1 x 104 cells/well are added to human L-Selectin coated plates (10 μg/ml with 100 μl/well), approximately 90 - 100% will adhere for 1 hour incubation at RT.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The extracellular domain of human L-Selectin (amino acid residues 1 - 332) (Siegelman, M.H. et al., 1989, Proc. Natl. Acad. Sci. USA 86:5562 - 5566; Tedder, T.F. et al., 1989, J. Exp. Med. 170:123 - 133; Camerini, D. et al., 1989, Nature 342:78 - 82) was fused to the carboxy-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker, inserted into a suitable mammalian expression vector and expressed in NSO cells.
References
1. Levy K. et al.(2005) J. Cell Mol. Med. 9: 255
2. Spangrude GJ. et al.(2004) Blood 103: 2990
Related Products
Recombinant Mouse L-Selectin (Leinco Prod. No.: L199)
Anti-Mouse L-Selectin (Leinco Prod. No.: L218)
Anti-Mouse L-Selectin (Antigen Aff. Pur.) (Leinco Prod. No.: L222)
Anti-Human L-Selectin (Antigen Aff. Pur.) (Leinco Prod. No.: L224)
Anti-Human L-Selectin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L233)
