产品编号:L199-100
产品描述:Recombinant Mouse L-Selectin
反应种属:
实验方法:
标记:
规格:100ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Mouse L-Selectin
Purified No Carrier Protein
(CD62L, SELL, LAM-1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc, MEL-14 Antigen, DREG)
Prod. No.: L199
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain1 found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.2
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse L-Selectin is Mr 60.5 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 150 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells. When 1 x 104 cells/well are added to L-Selectin coated plates approximately 90 - 100% will adhere for 1 hour incubation at RT.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
wt yhysekpmnw enarkfckqn ytdlvaiqnk reieylentl pkspyyywig irkigkmwtw vgtnktltke aenwgagepn nkkskedcve iyikrerdsg kwnddachkr kaalcytasc qpgscngrge cvetinnhtc icdagyygpq cqyvvqcepl eapelgtmdc ihplgnfsfq skcafncseg rellgtaetq cgasgnwssp epicqvvqce pleapelgtm dcihplgnfs fqskcafncs egrellgtae tqcgasgnws spepicqetn rsfskikegd yniegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh
References
1. Levy K. et al.(2005) J. Cell Mol. Med. 9: 255
2. Spangrude GJ. et al.(2004) Blood 103: 2990
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