产品编号:I-539-1
产品描述:Recombinant Human IgG
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Immunoglobulin G (IgG)
Purified No Carrier Protein
Prod. No.: I-539
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
Immunoglobulin G (IgG) is a monomeric immunoglobulin synthesized and secreted by plasma B cells. It is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids (1). IgG antibodies are predominately involved in the secondary antibody response (the main antibody involved in primary response is IgM) (2). Pro-inflammatory cytokines particularly IL-4 and IL-2 have a crucial role in activation of the IgG antibody response. IgG is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in utero (3). In addition, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IgG is Mr 26.6 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 30-35 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
This protein is meant for use as a control.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
iegrmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk
References
1. Junqueira, L. et al. (2003) Basic Histology. McGraw-Hill
2. Meulenbroek, A. J. et al. (1996) Human IgG Subclasses: Useful diagnostic markers for immunocompetence (Sanquin)
3. Pitcher-Wilmott, R. W. et al. (1980) Clin. Exp. Immunol. 41:303
