产品编号:I-811-2
产品描述:Recombinant Mouse IL-27 (p28)
反应种属:
实验方法:
标记:
规格:2ug
供应商:Leinco
价格(RMB):2295.00
订购:
说明书:
Recombinant Mouse Interleukin-27/p28 Subunit (IL-27)
Purified No Carrier Protein
(P28, IL30, IL-27, IL-27p28, IL27)
Prod. No.: I-811
Source E. coli
Pkg. Size: 1 mg, 2 µg, 10 µg, 100 µg
Storage: -20°C
Description
Background:
Interleukin-27 (IL-27) is a novel IL-12 family member that plays a role in the early regulation of T helper cell 1 initiation, including induction of T-bet and IL-12 receptor beta 2 expression.1 IL-27 is produced by antigen-presenting cells2 and plays an important function in regulating the activity of B and T lymphocytes.3 IL27 may be a potential therapeutic agent against rheumatoid arthritis (RA) at the onset of the disease.4
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse IL-27 is Mr 23.7 kDa.
State of Matter
Lyophilized
Purity
>97% by SDS-PAGE and HPLC
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse IL-27 was determined by dose-dependent inhibition of TGF-beta & IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50 ng/ml of mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS
References
1. Yoshimoto T et al. (2004) Cancer Res. 64: 1152
2. De Sauvage FJ et al. (2003) Proceedings of the National Acad of Sci of USA 100: 15047
3. Devergne O et al. (2006) J Immunol. 176: 5890
4. Liew FY et al. (2008) Ann Rheum Dis. 67: 1474
Related Products
Anti-Human IL-27 (Intermediate Domain) (Leinco Prod. No.: I-500)
Anti-Human IL-27 (NT) (Leinco Prod. No.: I-501)
IL-27 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: I-512)
IL-27 (NT) Blocking Peptide (Leinco Prod. No.: I-513)
