产品编号:I-230-100
产品描述:Recombinant Human IL-22
反应种属:
实验方法:
标记:
规格:100ug
供应商:Leinco
价格(RMB):11775.00
订购:
说明书:
Recombinant Human Interleukin-22 (IL-22)
Purified No Carrier Protein
(ZcytoR11, CRF2-9, IL-TIF)
Prod. No.: I-230
Source E. coli
Pkg. Size: 1 mg, 2 µg, 10 µg, 100 µg
Storage: -20°C to -70°C
Description
Background:
IL (interleukin)-22 is an IL-10 homologue, produced by activated T cells that binds to and signals via the class II cytokine receptor (R) heterodimer IL-22RA1/CFR2-4 (IL-10R2).1 IL-22 regulates the production of acute phase proteins of the immunological response. On binding to its cognate receptor (IL-22R1), which is associated to the interleukin-10 receptor 2, IL-22 promotes activation of signal transducer and activator of transcription (STAT) pathway and several other cellular responses.2 IL-22 plays an important role in skin inflammatory processes and wound healing.3
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human IL-22 is Mr 16.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS Page and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-22 was determined by its ability to induce IL-10 secretion in Colo205 cells (Nagalakshmi, M.L. et al., 2004, International Immunopharmacology. 4:679-691 The expected ED50 for this effect is typically 60-300 pg/mL.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the mature human IL-22 protein sequence (Ala 34 - Ile 179) was inserted into a suitable expression vector and was expressed in E coli (Dumoutier, L. et al., 2000, PNAS 97:10144).
References
1. Yssel H. et al.(2002) Int. Immunol. 14: 1351
2. Polikarpov I. et al.(2006) Vitam Horm. 74: 77
3. Morel F. et al. (2005) J. Immunol. 174: 3695
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