产品编号:I-452-10
产品描述:Recombinant Human IL-21
反应种属:
实验方法:
标记:
规格:10ug
供应商:Leinco
价格(RMB):3975.00
订购:
说明书:
Recombinant Human Interleukin-21 (IL-21)
Purified No Carrier Protein
(Za11)
Prod. No.: I-452
Source E. coli
Pkg. Size: 1 mg, 2 µg, 10 µg, 100 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin 21 (IL-21) is a T cell-derived pleiotropic cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells.1,2 This cytokine induces cell division/proliferation in its target cells. IL-21 elicits its effects on immune cells by interacting with a cell surface receptor known as the interleukin 21 receptor, IL-21R, which is expressed in bone marrow cells and various lymphocytes. IL-21 is closely related to IL-2 and IL-153 and is a key factor in the transition between innate and adaptive immune responses.4 The critical role of IL21 in promoting humoral immune responses makes it an important focus of potential therapeutic interventions in conditions characterised by overproduction of pathogenic autoantibodies.5
Source
E. coli
Molecular Weight
The molecular weight of Recombinant Human IL-21 is Mr 15.4 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS Page and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-21 was determined determined by the dose-dependant stimulation of human peripheral blood mononuclear cells. The expected ED50 is <5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
References
1. Foster D et al. (2000) Nature 408: 57
2. Clegg CH et al. (2002) J. Leukoc. Biol. 72: 856
3. Leonard WJ.et al. (2002) Science 298: 1630
4. Joannopoulos K.et al. (2003) J. Immunol. 171: 608
5. Lipsky PE. et al. (2008) Ann Rheum. Dis. 3: 83
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