产品编号:I-338-1
产品描述:Recombinant Human IL-20
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Interleukin-20 (IL-20)
Purified No Carrier Protein
(ZCYTO10, IL10D, MGC96907; IL20)
Prod. No.: I-338
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin-20 (IL-20) is a pleiotropic inflammatory protein belonging to the IL-10 family of cytokines. Members of this family share partial homology in their amino acid sequences but they are dissimilar in their biological functions. IL-20 is capable of stimulating colony formation by CD34+ multipotential progenitors but not by other progenitor cells (1). It is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis (2). Human IL-20 is active on mouse cells.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human IL-20 is Mr 17.6 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with BSA as a carrier protein. pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-20 was determined by its ability to induce proliferation of mouse BaF/3 cells co-transfected with human IL-20 Rα and IL-20 Rβ. The expected ED50 for this effect is typically 0.2 - 0.6 ng/mL.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mlktlnl gscviatnlq eirngfsdir gsvqakdgni dirilrrtes lqdtkpanrc cllrhllrly ldrvfknyqt pdhytlrkis slansfltik kdlrlchahm tchcgeeamk kysqilshfe klepqaavvk algeldillq wmeete
References
1. Kupper, T. S. et al. (2006) Anti-Inflamm. & Anti-Allergy Agents Med. Chem. 5:243
2. Dam, T. M. et al. (2009) Br. J. Dermatol. 160:284
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