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Recombinant Human IL-18 BPa/Fc

产品编号:I-546-1

产品描述:Recombinant Human IL-18 BPa/Fc

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:订购

说明书:

Recombinant Human Interleukin 18 Binding Protien a (IL-18 BPa)
Purified No Carrier Protein
(IL18BPa, IL18BP)
Prod. No.: I-546
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C

Description
Background:

IL-18-binding protein (IL-18BP) is a secreted glycoprotein in the IL-1 family of ligands, however, it bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. IL-18BP functions as a natural anti-inflammatory and immunosuppressive molecule neutralizing the effects of high IL-18 levels during inflammation (1). It is constitutively expressed and secreted in mononuclear cells and can be enhanced by IFN gamma, which suggests IL-18 activity is modulated by a negative feedback mechanism modulated by IL-18BP. There are four known variants of IL-18BP, isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain. IL18BPa is the predominant form showing the highest affinity towards IL-18 (2). IL-18 BPa:Fc has the potential to amplify anti-inflammatory actions of immunosuppressive drugs, and thus may prove to be a valuable novel pharmacological component in the treatment of human autoimmune diseases (3).

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-18 BPa is Mr 44.2 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 60-80 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence

tp vsqtttaata svrstkdpcp sqppvfpaak qcpalevtwp evevplngtl slscvacsrf pnfsilywlg ngsfiehlpg rlwegstsre rgstgtqlck alvleqltpa lhstnfscvl vdpeqvvqrh vvlaqlwagl ratlpptqea lpsshsspqq qgiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgk

References
1. Novick, D. et al. (1999) Immunity 10:127
2. Kim, S. H. et al. (2000) Proc. Nat. Acad. Sci. (USA) 197:1190
3. Mühl, H. et al. (2003) Biochemical pharmacology 66:505

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