产品编号:I-197-10
产品描述:Recombinant Human IL-16
反应种属:
实验方法:
标记:
规格:10ug
供应商:Leinco
价格(RMB):3735.00
订购:
说明书:
Recombinant Human Interleukin-16 (IL-16)
Purified No Carrier Protein
(FLJ16806, HsT19289, Lymphocyte Chemoattractant Factor (LCF), PrIL-16)
Prod. No.: I-197
Source E. coli
Pkg. Size: 1 mg, 2 µg, 10 µg, 100 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin 16 (lymphocyte chemoattractant factor), also known as IL16, is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. IL-16 is released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. IL-16 has a critical role in protecting antigen presenting cells (APC) against HIV-1 infection and is used in the treatment of HIV disease.1
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human IL-16 is Mr 14 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>95% by SDS-PAGE and HPLC
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-16 (130 a.a.) has not been determined. The expected ED50 in a biological assay has not been determined. However, human IL-16 may be used as a standard in an ELISA assay to create a standard curve.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the putative 130 aa residue IL-16 monomer was inserted into a suitable expression vector and was expressed in E. coli (Cruikshank, W.W. et al., 1994, Proc. Natl. Acad. Sci. USA 91:5109 - 5113).
References
1. Bahr GM. et al. (1999) J. Virol. 73: 7008
Related Products
Anti-Human IL-16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-271)
Anti-Human IL-16 (Antigen Aff. Pur.) (Leinco Prod. No.: I-158)
Recombinant Human IL-16 (121 a.a.) (Leinco Prod. No.: I-336)
Anti-Human IL-16 (Leinco Prod. No.: I-654)
Recombinant Mouse IL-16 (Leinco Prod. No.: I-810)
