产品编号:I-549-100
产品描述:Recombinant Human IL-15 Rα
反应种属:
实验方法:
标记:
规格:100ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Human Interleukin-15 Receptor Alpha (IL-15 Rα)
Purified No Carrier Protein
(IL15RA, MGC104179)
Prod. No.: I-549
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor. IL15RA is a cytokine receptor that specifically binds IL15 with high affinity. The receptors of IL15 and IL2 share two subunits, the IL2R beta and IL2R gamma chains. This forms the basis of many overlapping biological activities of IL15 and IL2. IL15RA is structurally related to IL2R alpha, but is capable of binding IL15 with high affinity independent of other subunits, which suggests the distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2.
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-15 Rα/Fc is Mr 42.6 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 60-70 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS Page and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-15 Rα was determined by its ability to block human IL-15 induced CTLL-2 proliferation. The expected ED50 for this effect is typically 0.005 - 0.015 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the extracellular domain of human IL-15 Rα ΔE3, which lacks exon 3, (Anderson, D. et al., 1995, J. Biol. Chem. 270:29862 - 29869) was fused to the 6X histidine tagged Fc of human IgG1 and was expressed in Sf 21 cells using a baculovirus expression system
Related Products
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