产品编号:I-196-25
产品描述:Recombinant Human IL-15
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):9900.00
订购:
说明书:
Recombinant Human Interleukin-15 (IL-15)
Purified No Carrier Protein
(IL-T, MGC9721)
Prod. No.: I-196
Source E. coli
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin-15 (IL-15), also known as IL-T and MGC9721, is an immunomodulating cytokine that stimulates the proliferation of T lymphocytes and shares many biological properties with IL-2 (1). IL-15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta and skeletal muscle. It exerts its biological activities primarily on T cells and is essential in the development, survival and activation of NK cells (2). IL-15 is also secreted by activated monocytes/macrophages following infection by virus (3). It competes for binding sites with IL-2, as both stimulate the growth of cells through the IL-2 receptor and thus negatively regulate each others activity (4). IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. It can recruit and activate T lymphocytes in the synovial membrane, thereby contributing to Rheumatoid arthritis pathogenesis (5). Increased expression of IL-15 has also been implicated with inflammatory bowl disease and diseases affiliated with retroviruses HIV and HTLV-I (6). Human IL-15 is biologically active on mouse cells as measured by the dose-dependent stimulation of the proliferation of mouse CTLL cells.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human IL-15 is Mr 12.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-15 was determined in a cell proliferation assay using an IL-15 responsive human megakaryocytic leukemic cell line, MO7e. The expected ED50 for this effect is typically 0.8 - 4 ng/ml. The cell number is assessed in a fluorometric assay using the redox sensitive dye, Resazurin
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
nw vnvisdlkki edliqsmhid atlytesdvh psckvtamkc fllelqvisl esgdasihdt venliilann slssngnvte sgckeceele eknikeflqs fvhivqmfin ts
References
1. Grabstein, K. et al. (1994) Science 264:965
2. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657
3. Yoshikai, Y. et al. (1996) J. Immunol. 156:663
4. Giri, J. et al. (1994) EMBO J. 13:2822
5. Liew, F. Y. et al. (1996) Nat. Med. 4:643
6. Waldmann, T. A. et al. (1999) Annu. Rev. Immunol. 17:19
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